Papanicolaou solution 1B 1x2500 ml

Papanicolaou solution 1B 1x2500 ml

Papanicolaous solutions,
Haematoxylin solutions
Staining solutions and colourants for cytological cancer and cycle diagnostics
Concept
The papanicolaous staining is the most used staining method for
cytological material.
First, the cores are stained with a haematoxylin solution. The cores appear blue, dark violet or black
The second step isthe cytoplasm staining with an orange staining solution which shows especially the mature and cornified cells. The target structures are stained in orange of different intensities
In the third staining step, the polychrome solution is used. It is a mix of eosin, light green SF and Bismarck brown. With the polychrome solution, the
differentiationof the squamous epithelium is shown.
Application:
The haematoxylin colourants are mixed with trivalent metal salts and
create what is referred to as haematoxylin lacquers, which are used selectively for staining the
cores (DNA). Haematoxylin or, better, haematein creates - together with
the respective metal ions of the alums (Aluminium, Iron or Chrome) -
complex compounds, chelate compounds that are used
in the acidic environment and which show the characteristic blue colour due to rinsing with
tap water what is referred to as blueing. This stepalso fixated
the colour to the target structures.
Two types are distinguished: the progressive haematoxylin staining, when staining takesplace until
the end point and then, the tap water is made blue and durable.
And the regressive method is over-staining with haematoxylin and the excessive
colour is removed in acidic differentiation steps. Here also, it is made blue with tap water and the staining is made durable.
For the regressive staining, the core structures are more differentiated and
more visible.